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♪ Large cattle
PCR

PCR-BOVINE-LUMPY-SKIN-DISEASE-FAKTOR (cat. no. D21416-VET) (55 reactions / 110 reactions)

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Intended use

Reagent kit to detect DNA noduular dermatite virus (Lumpy skin dissease virus, LSDV) in biological material (fragments of affected skin cover, organs, whole blood, mucous concussive and rotor throat swabs, milk, sperm)

Catalogue number
D21416-VET
Method / type
PCR
Section
Cattle
Animal species
Large cattle, goats and sheep

Specifications

Compilation

55 reactions / 110 reactions, including controls (COE, COE, DOC).
The use requires a set of reagents to extract NC (e.g. DNA/RNA-C-ACTOR).

Storage conditions

The reagent kit consists of 2 components:

  • Set No. 1 from -20 to -18°C;

  • Set No. 2 from 2 to 8 °C

Shelf life

12 months

Description

The reagent kit is designed to detect DNA nodular dermatite virus (Lumpy skin disase virus, LSDV) in biological material (fragments of affected skin cover, organs, whole blood, mucous concussive and rot throat swabs, milk, sperm) using a polymerase chain reaction (PCR) with a fluorescent detachment in real time.

Nodular (communicable knot) dermatitis is a viral disease of cattle and buffalo. The agent is from Capripoxvirus, which also includes sheep smallpox and goats. The disease is characterized by fever, dense knots on the skin, increased surface lymph nodes, mucous shells, edema and diarrhea. The virus is carried by blood-sucking insects and mites, and direct contact between animals plays a smaller role. The disease in the herd is usually between 5 and 45 per cent, with mortality generally below 10 per cent. The main damage is reduced productivity, skin damage, abortion and restrictions on the movement of livestock. lumpy skin disease is included in the list of diseases of the World Organisation for Animal Health (WOAH), previously OIE, that countries are required to report.

Symptoms of noduular dermatitis are similar to the expressions of pseudonodular dermatitis (type 2 herpesvirus CRS), dermatophilosis, demodecosis, non-noitiasis, prunes and insect bites, so the diagnosis is confirmed laboratoryly. The main method of confirmation recommended by the WWD is the PCR.


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