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PCR

PCR-COCCIDIOSIS-FAKTOR (cat. no. D55826-VET) (55 reactions / 110 reactions)

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Intended use

The kit is intended for the detection of DNA of protozoa of the family Eimeriidae that cause coccidiosis in biological material.
Catalogue number
D55826-VET
Method / type
PCR
Section
Poultry
Animal species
Poultry

Specifications

Product information

55 reactions / 110 reactions, controls included (internal, positive and negative).
A nucleic acid extraction kit is required (for example, DNA/RNA-S-FAKTOR).

Description

The kit is intended for the detection of DNA of protozoa of the family Eimeriidae (including E. tenella, E. maxima, E. acervulina, E. praecox, E. mitis, E. brunetti, E. necatrix and others) that cause coccidiosis in biological material (whole blood, faeces, droppings, parenchymal organs and lymph nodes), as well as in food and feed of animal and plant origin, microbial cultures and surface swabs, by DNA amplification with real-time fluorescence detection.

Order questions

How to obtain a commercial offer?

Add the necessary goods to the KP request, specify the quantity and contacts. The specialist will clarify the conditions of delivery.

Can we discuss the task of the lab?

Leave a comment on the request or order a return call.

How do I get in touch with the company?

Phone: +7 (495) 789-48-11B. Mail: vet@faktor.ru. Office hours: weekdays, 08:00–16:30.

About the disease

Coccidiosis

Pathogens: protozoa of the family Eimeriidae, above all Eimeria

A bit of history

The genus Eimeria was named in 1875 after the German zoologist Theodor Eimer, who studied these parasites. Eimeria tenella in chickens was described in 1891, and in 1929 the American pathologist Ernest Tyzzer sorted out several coccidian species of domestic poultry. Anticoccidial drugs have been used since the 1940s, and live vaccines appeared later.

Who is affected

Each animal species has its own strictly host-specific Eimeria: seven species have long been known in chickens, the most dangerous being E. tenella and E. necatrix, and three more were described in 2021. Young animals suffer most, especially broiler chicks in their first weeks of life. The parasites destroy the intestinal lining and birds gain weight poorly, which is why coccidiosis is considered one of the costliest poultry diseases in the world.

How the diagnosis is confirmed in Russia

Coccidiosis is diagnosed from clinical signs, intestinal lesions at necropsy and faecal examination: oocysts in droppings are detected and counted by flotation methods. Eimeria species are hard to tell apart by their oocysts. PCR detects Eimeria DNA in droppings, organs and feed, and species-specific PCR assays make it possible to identify the species precisely.

For reference only. The diagnosis is made by a veterinary specialist based on laboratory test results.

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