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ELISA

ID Screen® Influenza A Nucleoprotein Indirect

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Intended use

The only commercially available indirect ELISA to detect antibodies for the nucleoproteid (NP) virus in chicken and turkey blood serum.
Catalogue number
FLUNPS
Method / type
ELISA
Section
Gripp A/ Gripp Birds
Animal species
Poultry

Description

The only commercially available indirect ELISA for detecting antibodies to viral nucleoprotein (NP) in chicken and turkey serum.

  • Developed for monitoring inactivated vaccines in chickens and turkeys
  • Can be used to differentiate between vaccinated and infected animals (DIVA*) together with the ID Screen competitive ELISA for influenza H5 antibodies, to detect natural infection in animals vaccinated with rHVT-H5 vaccines
  • Feasibility of using a test system for disease monitoring in specific-pathogen-free (SPF) laboratory animals (similar to the ID Screen set for competitive multi-species ELISA for anti-nucleoprotein (NP) antibodies) due to high sensitivity
  • High correlation with the haemagglutination inhibition test
  • Quantitative analysis: results can be expressed as titres
  • Integrated data analysis using IDSoft™ software
  • A methodology is expected for the study of egg yolk of hens without chloroform. Easier sample preparation for veterinarians and reduced stress in animals

*Differentiating infected from vaccinated animals

TECHNICAL CHARACTERISTICS

Assay method

Indirect ELISA

Species

Chickens (broilers, breeders and layers) and turkeys

Sample types

Serum or plasma

The egg yolk testing protocol is undergoing validation; please enquire

Coated antigen

NP Recombinant protein

Conjugate

Anti-chicken-HRP conjugate (concentration 10X)

ORDERING INFORMATION

Product code

FLUNPS-5P

FLUNPS-10P

Kit format

5 plates

10 plates

Number of reactions

480

960

Plate format

12 strips of 8 wells

12 strips of 8 wells

REFERENCE SERA

Ready-to-use negative chicken serum

Product code

MRINEG-BIRD-RTU

Presentation

5 ml vial

Description

Ready-to-use pooled specific-pathogen-free (SPF) chicken serum.

Does not contain antibodies to avian pathogens.

For use with all IDvet chicken ELISA kits or rapid slide agglutination (RSA)

Freeze-dried negative specific-pathogen-free (SPF) serum

Product code

MRINEG-BIRD

Presentation

0.5 ml vial (freeze-dried)

Description

Freeze-dried negative specific-pathogen-free (SPF) chicken serum for use as an internal reference material for quality control.

For use in ELISA or rapid slide agglutination (RSA)

Order questions

How to obtain a commercial offer?

Add the necessary goods to the KP request, specify the quantity and contacts. The specialist will clarify the conditions of delivery.

Can we discuss the task of the lab?

Leave a comment on the request or order a return call.

How do I get in touch with the company?

Phone: +7 (495) 789-48-11B. Mail: vet@faktor.ru. Office hours: weekdays, 08:00–16:30.

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